pLp6.3-EglN3-H196A
(Plasmid
#79116)
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PurposeLentiviral expression of EglN3-H196A
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 79116 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti6.3
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 7692
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEglN3 catalytic dead mutant
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Alt namePHD3 mutant
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SpeciesH. sapiens (human)
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Insert Size (bp)720
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Mutationcatalytic dead mutant: H196A
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Entrez GeneEGLN3 (a.k.a. HIFP4H3, HIFPH3, PHD3)
- Promoter CMV
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Tag
/ Fusion Protein
- V5 (C terminal on backbone)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLp6.3-EglN3-H196A was a gift from Susanne Schlisio (Addgene plasmid # 79116 ; http://n2t.net/addgene:79116 ; RRID:Addgene_79116)