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Addgene

pLp6.3-EglN3-H196A
(Plasmid #79116)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79116 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLenti6.3
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 7692
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EglN3 catalytic dead mutant
  • Alt name
    PHD3 mutant
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    720
  • Mutation
    catalytic dead mutant: H196A
  • Entrez Gene
    EGLN3 (a.k.a. HIFP4H3, HIFPH3, PHD3)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLp6.3-EglN3-H196A was a gift from Susanne Schlisio (Addgene plasmid # 79116 ; http://n2t.net/addgene:79116 ; RRID:Addgene_79116)