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Addgene

GFP-KIF1B beta-FL
(Plasmid #79108)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79108 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP-KIF1B beta full length
  • Alt name
    kinesin family member 1B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5780
  • Mutation
    exon 25 missing (natural splice varient)
  • Entrez Gene
    KIF1B (a.k.a. CMT2, CMT2A, CMT2A1, HMSNII, KLP, NBLST1)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-KIF1B beta-FL was a gift from Susanne Schlisio (Addgene plasmid # 79108 ; http://n2t.net/addgene:79108 ; RRID:Addgene_79108)

  • For your References section:

    RNA helicase A is a downstream mediator of KIF1Bbeta tumor-suppressor function in neuroblastoma. Chen ZX, Wallis K, Fell SM, Sobrado VR, Hemmer MC, Ramskold D, Hellman U, Sandberg R, Kenchappa RS, Martinson T, Johnsen JI, Kogner P, Schlisio S. Cancer Discov. 2014 Apr;4(4):434-51. doi: 10.1158/2159-8290.CD-13-0362. Epub 2014 Jan 27. 10.1158/2159-8290.CD-13-0362 PubMed 24469107
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