psfTn5
(Plasmid #79107)

• Purpose: bacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutations
• Depositing Lab: Sten Linnarsson
• Publication: Linnarsson lab plasmids (unpublished)

Backbone

• Vector backbone: pNIC-Bsa4
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: transposase 5
• Alt name: Tn5
• Mutation: E54K and L372P
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminal

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Plasmid was cloned by the Protein Science Facility at Karolinksa Institute
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry