pWPI-myogenin
(Plasmid
#79073)
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PurposeLentiviral plasmid that constitutively expresses human myogenin
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 79073 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepWPI
- Backbone size w/o insert (bp) 11001
- Total vector size (bp) 11804
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman myogenin
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SpeciesH. sapiens (human)
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Insert Size (bp)672
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Entrez GeneMYOG (a.k.a. MYF4, bHLHc3, myf-4)
- Promoter EF1 alpha
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (unknown if destroyed)
- 3′ cloning site NdeI (unknown if destroyed)
- 5′ sequencing primer pWPI upstream primer
- 3′ sequencing primer pWPI downstream primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWPI-myogenin was a gift from Jennifer Lei (Addgene plasmid # 79073 ; http://n2t.net/addgene:79073 ; RRID:Addgene_79073)