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Addgene

pCI-ING1a
(Plasmid #79069)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79069 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4006
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ING1 (p47 ING1A isoform)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1266
  • GenBank ID
    AF181849.1
  • Entrez Gene
    ING1 (a.k.a. p24ING1c, p33, p33ING1, p33ING1b, p47, p47ING1a)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-ING1a was a gift from Karl Riabowol (Addgene plasmid # 79069 ; http://n2t.net/addgene:79069 ; RRID:Addgene_79069)

  • For your References section:

    ING1a expression increases during replicative senescence and induces a senescent phenotype. Soliman MA, Berardi P, Pastyryeva S, Bonnefin P, Feng X, Colina A, Young D, Riabowol K. Aging Cell. 2008 Dec;7(6):783-94. doi: 10.1111/j.1474-9726.2008.00427.x. Epub 2008 Sep 16. 10.1111/j.1474-9726.2008.00427.x PubMed 18691180
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