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CSII-IDR2-humanTRIM5-FOS
(Plasmid #79066)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79066 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CSII-BBN-IRES-DsRed
  • Backbone manufacturer
    CSII-BBN-IDR2
  • Backbone size w/o insert (bp) 9866
  • Total vector size (bp) 11411
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TRIM5alpha(human)-FOS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1479
  • GenBank ID
    NP_149023.2
  • Entrez Gene
    TRIM5 (a.k.a. RNF88, TRIM5alpha)
  • Promoter CMV
  • Tag / Fusion Protein
    • OneSTrEP-FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer CAGAGCTGGTTTAGTGAACC
  • 3′ sequencing primer GCCAAAAGACGGCAATATGG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CMV promoter and IRES DsRed co expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CSII-IDR2-humanTRIM5-FOS was a gift from Wesley Sundquist (Addgene plasmid # 79066 ; http://n2t.net/addgene:79066 ; RRID:Addgene_79066)

  • For your References section:

    Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids. Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, Sundquist WI. Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269. 10.7554/eLife.16269 PubMed 27253068
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