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pBABE-3xFLAG-OB D166R
(Plasmid #79061)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79061 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-3xFLAG
  • Backbone manufacturer
    Franklin Zhong
  • Modifications to backbone
    Puro selection
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TPP1
  • Alt name
    TPP1-OB 166RR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    908
  • Mutation
    Aspartic acid 166 changed to Arginine
  • Entrez Gene
    TPP1 (a.k.a. CLN2, GIG1, LPIC, SCAR7, TPP-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TPP1-OB insert was cloned using unique restriction sites, AscI and PacI into a custom-made pBABE-3xFLAG-NLS vector. Epitope tag was part of the vector backbone. See attached files for the complete sequence from the initiating ATG to the stop codon of the 3xFLAG-NLS-TPP1-OB ORF

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-3xFLAG-OB D166R was a gift from Steven Artandi (Addgene plasmid # 79061 ; http://n2t.net/addgene:79061 ; RRID:Addgene_79061)

  • For your References section:

    TPP1 OB-fold domain controls telomere maintenance by recruiting telomerase to chromosome ends. Zhong FL, Batista LF, Freund A, Pech MF, Venteicher AS, Artandi SE. Cell. 2012 Aug 3;150(3):481-94. doi: 10.1016/j.cell.2012.07.012. 10.1016/j.cell.2012.07.012 PubMed 22863003
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