IMS-APEX2
(Plasmid #79058)

• Purpose: Expresses APEX in the mitochondrial intermembrane space.
• Depositing Lab: Alice Ting
• Publication: Hung et al Mol Cell. 2014 Jul 17;55(2):332-41. doi: 10.1016/j.molcel.2014.06.003. Epub 2014 Jul 4.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5595
• Total vector size (bp): 6390
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: APEX
• Species: Soybean
• Insert Size (bp): 795
• Promoter: T7 promoter
• Tag / Fusion Protein:
  • V5 (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 promoter primer

Resource Information

• Supplemental Documents:
  • ApE file for plasmid
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry