MICU-APEX2
(Plasmid #79057)

• Purpose: A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1
• Depositing Lab: Alice Ting
• Publication: Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.

Backbone

• Vector backbone: pLX304
• Backbone size w/o insert (bp): 7740
• Total vector size (bp): 10029
• Vector type: Mammalian Expression, Bacterial Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: C-terminal APEX2 fused MICU1
• Species: H. sapiens (human); Soybean
• Insert Size (bp): 2289
• Entrez Gene: MICU1 (a.k.a. CALC, CBARA1, EFHA3, MPXPS, ara CALC)
• Promoter: CMV immearly promotor
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-forward

Resource Information

• Supplemental Documents:
  • ApE file for plasmid
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry