ERM-APEX2
(Plasmid #79055)

• Purpose: Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.
• Depositing Lab: Alice Ting
• Publication: Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.

Backbone

• Vector backbone: pLX304
• Backbone size w/o insert (bp): 7740
• Total vector size (bp): 8694
• Vector type: Mammalian Expression, Bacterial Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: APEX2 anchored to the cytosolic face of the ER membrane
• Species: Soybean
• Insert Size (bp): 954
• Promoter: CMV immearly promotor
• Tags / Fusion Proteins:
  • Residues 1-27 of P450 oxidase 2C1 (N terminal on insert)
  • V5 (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-forward

Resource Information

• Supplemental Documents:
  • ApE file for plasmid
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Additional publication for this plasmid:
Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation.
Hung et. al. Elife 2017 https://www.ncbi.nlm.nih.gov/pubmed/28441135