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Addgene

pSpyTag-GlpG
(Plasmid #79054)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79054 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTrcHisB
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4269
  • Total vector size (bp) 4992
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SpyTag-GlpG
  • Insert Size (bp)
    723
  • Mutation
    GlpG protein employed here corresponds to residues 87-276 of E. coli GlpG.
  • Entrez Gene
    glpG (a.k.a. b3424, ECK3410)
  • Promoter Trc
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • SpyTag (N terminal on insert)
    • SpyTag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13pUC-Rev
  • 3′ sequencing primer pBAD-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Transform pSpyTag-GlpG plasmid into E. coli BL21-Gold (DE3) for expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSpyTag-GlpG was a gift from James Bowie (Addgene plasmid # 79054 ; http://n2t.net/addgene:79054 ; RRID:Addgene_79054)

  • For your References section:

    A simple DNA handle attachment method for single molecule mechanical manipulation experiments. Min D, Arbing MA, Jefferson RE, Bowie JU. Protein Sci. 2016 Aug;25(8):1535-44. doi: 10.1002/pro.2952. Epub 2016 Jun 6. 10.1002/pro.2952 PubMed 27222403
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