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pFastBac1-Kozak-rhTRIM5alpha-PFOS
(Plasmid #79038)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79038 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac1
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 4775
  • Total vector size (bp) 6434
  • Vector type
    Insect Expression
  • Selectable markers
    Ampicillin

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    requires transformation into E. coli DH10Bac for bacmid production
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rhesus TRIM5alha-PFOS
  • Alt name
    rhTRIM5alpha-PFOS
  • Species
    Macaca mulatta
  • Insert Size (bp)
    1653
  • GenBank ID
    DQ842021.1
  • Promoter polyhedrin
  • Tag / Fusion Protein
    • OneSTrEP-FLAG (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer ATACCGTCCCACCATCG
  • 3′ sequencing primer GATCCTCTAGTACTTCTCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositor-provided reference sequence shows M221T and P307T compared to DQ842021.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac1-Kozak-rhTRIM5alpha-PFOS was a gift from Wesley Sundquist (Addgene plasmid # 79038 ; http://n2t.net/addgene:79038 ; RRID:Addgene_79038)

  • For your References section:

    Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids. Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, Sundquist WI. Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269. 10.7554/eLife.16269 PubMed 27253068
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