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pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)
(Plasmid #79036)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79036 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac1
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 4775
  • Total vector size (bp) 6381
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin ; Ampicillin

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    requires transformation into E. coli DH10Bac for bacmid production
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OSF-TRIM5Cyp (owl monkey) (K283D, Q287D)
  • Species
    Aotus trivirgatus
  • Insert Size (bp)
    1587
  • Mutation
    Changed Lys 283 to Asp and Gln 287 to Asp
  • GenBank ID
    AAT73777.1
  • Promoter polyhedrin
  • Tag / Fusion Protein
    • OneSTrEP-FLAG (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer ATACCGTCCCACCATCG
  • 3′ sequencing primer GATCCTCTAGTACTTCTCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D) was a gift from Wesley Sundquist (Addgene plasmid # 79036 ; http://n2t.net/addgene:79036 ; RRID:Addgene_79036)

  • For your References section:

    Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids. Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, Sundquist WI. Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269. 10.7554/eLife.16269 PubMed 27253068
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