Skip to main content
Addgene

pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)
(Plasmid #79031)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79031 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pFastBac1
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 4775
  • Total vector size (bp) 6507
  • Vector type
    Insect Expression
  • Selectable markers
    Ampicillin

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    requires transformation into E. coli DH10Bac for bacmid production
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OSF-TRIM5alpha(African green monkey pygerythrus)
  • Alt name
    OSF-TRIM5alpha(AGMpyg)
  • Species
    Chlorocebus pygerythrus pygerythrus
  • Insert Size (bp)
    1710
  • GenBank ID
    AAW72440.1
  • Promoter polyhedrin
  • Tag / Fusion Protein
    • OneSTrEP-FLAG (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer ATACCGTCCCACCATCG
  • 3′ sequencing primer GATCCTCTAGTACTTCTCG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that the TRIM5 insert in this plasmid contains a V434A polymorphism compared to GenBank ID AAW72440.1. Please see the full plasmid sequence for the most accurate information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus) was a gift from Wesley Sundquist (Addgene plasmid # 79031 ; http://n2t.net/addgene:79031 ; RRID:Addgene_79031)

  • For your References section:

    Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids. Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, Sundquist WI. Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269. 10.7554/eLife.16269 PubMed 27253068
Related items:
Commonly requested with: