pREP81xN_ccdB
(Plasmid
#79025)
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Purpose(Empty Backbone) Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selection
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 79025 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepREP-X
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Backbone manufacturerBasi, et al., PMID 8422997
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
MCS is in front of attR1 element and contains SpeI and NdeI restriction sites. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. MCS-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pREP81xN_ccdB was a gift from Göran Akusjärvi & Pernilla Bjerling (Addgene plasmid # 79025 ; http://n2t.net/addgene:79025 ; RRID:Addgene_79025)
Map uploaded by the depositor.
