pREP41xN_ccdB
(Plasmid #79023)

• Purpose: (Empty Backbone) Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selection
• Depositing Labs: Göran Akusjärvi, Pernilla Bjerling
• Publication: Akusjarvi and Bjerling plasmids (unpublished)

Backbone

• Vector backbone: pREP-X
• Backbone manufacturer: Basi, et al., PMID 8422997
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Resource Information

• Supplemental Documents:
  • Annotated sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

MCS is in front of attR1 element and contains SpeI restriction site.

Please note- the NdeI site has been disrupted in this plasmid. The NdeI site (CATATA instead of CATATG) will generate His-Ile instead of His-Met upon translation to protein. This region is linker between N-tag and protein of interest, so this should not affect protein function.

nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. MCS-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites.