pREP82xV5_ccdB
(Plasmid
#79020)
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Purpose(Empty Backbone) Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selection
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 79020 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepREP-X
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Backbone manufacturerBasi, et al., PMID 8422997
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Vector typeYeast Expression
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Selectable markersURA4+
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Tag
/ Fusion Protein
- double V5 epitope tag (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- double V5 epitope tag (N terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
NdeI site between V5 tag and attR1 element can be used to introduce additional tag(s) by conventional cloning. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. 2xV5-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pREP82xV5_ccdB was a gift from Göran Akusjärvi & Pernilla Bjerling (Addgene plasmid # 79020 ; http://n2t.net/addgene:79020 ; RRID:Addgene_79020)
Map uploaded by the depositor.
