pCIP-Flag-TfebAA
(Plasmid #79014)

• Purpose: For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.
• Depositing Lab: Reuben Shaw
• Publication: Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi: 10.1101/gad.274142.115.

Backbone

• Vector backbone: pCIP
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tfeb
• Species: M. musculus (mouse)
• Insert Size (bp): 1500
• Mutation: S142A, S211A
• Entrez Gene: Tfeb (a.k.a. Tcfeb, bHLHe35)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: MfeI (not destroyed)
• 5′ sequencing primer: pCAGGS-F1

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Depositor states that numbering of mutations may vary depending on the isoform in question and confirms that this plasmid should function as described.