pCIP-caTfeb
(Plasmid #79013)

• Purpose: For mammalian expression of a shortened, constitutively active version of Tfeb.
• Depositing Lab: Reuben Shaw
• Publication: Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi: 10.1101/gad.274142.115.

Backbone

• Vector backbone: pCIP
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tfeb
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Mutation: Removed first ~400 bp from 5' end (N-terminus)
• Entrez Gene: TFEB (a.k.a. ALPHATFEB, BHLHE35, TCFEB)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: MfeI (not destroyed)
• 5′ sequencing primer: pCAGGS F1

Resource Information

• A portion of this plasmid was derived from a plasmid made by: PCR'd Tfeb from: pegfp-N1-TFEB-S3A,R4A (Plasmid #44446)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry