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pDEST-hisMBP-AsCpf1-EC
(Plasmid #79007)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79007 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST-hisMBP
  • Backbone manufacturer
    Waugh Lab (addgene plasmid #11085)
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 10000
  • Modifications to backbone
    inserted E.coli codon optimized AsCpf1 coding sequence
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AsCpf1
  • Alt name
    AsCas12a
  • Species
    Synthetic
  • Insert Size (bp)
    4000
  • Promoter tac promoter
  • Tags / Fusion Proteins
    • 6xHis-MBP-NLS (N terminal on backbone)
    • NLS-HA (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-hisMBP-AsCpf1-EC was a gift from Jin-Soo Kim (Addgene plasmid # 79007 ; http://n2t.net/addgene:79007 ; RRID:Addgene_79007)

  • For your References section:

    Targeted mutagenesis in mice by electroporation of Cpf1 ribonucleoproteins. Hur JK, Kim K, Been KW, Baek G, Ye S, Hur JW, Ryu SM, Lee YS, Kim JS. Nat Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3596. 10.1038/nbt.3596 PubMed 27272385
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