RF-118-UB-BRCA1
(Plasmid #78960)

• Purpose: Expression of the C-terminal fragment of BRCA1 that is endogenously generated by caspase cleavage in mammalian cells
• Depositing Lab: Richard Fahlman
• Publication: Xu et al J Biol Chem. 2012 Mar 2;287(10):7495-502. doi: 10.1074/jbc.M111.301002. Epub 2012 Jan 18.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5600
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BRCA1
• Species: H. sapiens (human)
• Insert Size (bp): 2556
• Mutation: delta 1-1155
• Entrez Gene: BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
• Promoter: CMV
• Tags / Fusion Proteins:
  • ubiquitin (N terminal on backbone)
  • 3x Flag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry