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Purpose(Empty Backbone) Cloning vector for expression of LbCpf1 crRNA. It contains BsmB1 site for cloning.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78957 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19
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Modifications to backboneBsa1 site in the ampicillin-resistant gene was mutated.
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Vector typeMammalian Expression, CRISPR
- Promoter U6 promoter
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pU6-Lb-crRNA was a gift from Jin-Soo Kim (Addgene plasmid # 78957 ; http://n2t.net/addgene:78957 ; RRID:Addgene_78957) -
For your References section:
Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells. Kim D, Kim J, Hur JK, Been KW, Yoon SH, Kim JS. Nat Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3609. 10.1038/nbt.3609 PubMed 27272384
Map uploaded by the depositor.
