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pEGFP-RNF138-delta 158-180
(Plasmid #78923)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78923 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RNF138
  • Species
    H. sapiens (human)
  • Mutation
    delta 159-180
  • Entrez Gene
    RNF138 (a.k.a. HSD-4, NARF, STRIN, hNARF)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-RNF138-delta 158-180 was a gift from Michael Hendzel (Addgene plasmid # 78923 ; http://n2t.net/addgene:78923 ; RRID:Addgene_78923)

  • For your References section:

    The RNF138 E3 ligase displaces Ku to promote DNA end resection and regulate DNA repair pathway choice. Ismail IH, Gagne JP, Genois MM, Strickfaden H, McDonald D, Xu Z, Poirier GG, Masson JY, Hendzel MJ. Nat Cell Biol. 2015 Nov;17(11):1446-57. doi: 10.1038/ncb3259. Epub 2015 Oct 26. 10.1038/ncb3259 PubMed 26502055
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