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Addgene

HA-BAP1-N102S-GFP
(Plasmid #78915)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78915 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BAP1
  • Species
    H. sapiens (human)
  • Mutation
    N102S
  • Entrez Gene
    BAP1 (a.k.a. HUCEP-13, KURIS, TPDS1, UBM2, UCHL2, UVM2, hucep-6)
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequence results found an additional S558N mutation in BAP1 compared to GenBank reference sequence NP_004647.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-BAP1-N102S-GFP was a gift from Michael Hendzel (Addgene plasmid # 78915 ; http://n2t.net/addgene:78915 ; RRID:Addgene_78915)

  • For your References section:

    Germline mutations in BAP1 impair its function in DNA double-strand break repair. Ismail IH, Davidson R, Gagne JP, Xu ZZ, Poirier GG, Hendzel MJ. Cancer Res. 2014 Aug 15;74(16):4282-94. doi: 10.1158/0008-5472.CAN-13-3109. Epub 2014 Jun 3. 10.1158/0008-5472.CAN-13-3109 PubMed 24894717
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