HA-BAP1-F81V-GFP
(Plasmid
#78912)
-
PurposeMammalian expression of BAP1 with F81V and an EGFP fusion
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 78912 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepEGFP-N1
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameBAP1
-
SpeciesH. sapiens (human)
-
MutationF81V
-
Entrez GeneBAP1 (a.k.a. HUCEP-13, KURIS, TPDS1, UBM2, UCHL2, UVM2, hucep-6)
- Promoter CMV
-
Tags
/ Fusion Proteins
- HA (N terminal on insert)
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
HA-BAP1-F81V-GFP was a gift from Michael Hendzel (Addgene plasmid # 78912 ; http://n2t.net/addgene:78912 ; RRID:Addgene_78912) -
For your References section:
Germline mutations in BAP1 impair its function in DNA double-strand break repair. Ismail IH, Davidson R, Gagne JP, Xu ZZ, Poirier GG, Hendzel MJ. Cancer Res. 2014 Aug 15;74(16):4282-94. doi: 10.1158/0008-5472.CAN-13-3109. Epub 2014 Jun 3. 10.1158/0008-5472.CAN-13-3109 PubMed 24894717