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pWalium20-10XUAS-3XFLAG-dCas9-VPR
(Plasmid #78897)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78897 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWalium20
  • Backbone manufacturer
    Perrimon Lab
  • Backbone size w/o insert (bp) 9690
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9-VPR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5850
  • Promoter 10XUAS
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AAACATCCCATATTCAGCCGC
  • 3′ sequencing primer TTTGTCCAATTATGTCACAC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    dCas9-VPR insert cloned from PMID 25730490 into pWalium20 for in vivo Drosophila expression
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWalium20-10XUAS-3XFLAG-dCas9-VPR was a gift from Norbert Perrimon (Addgene plasmid # 78897 ; http://n2t.net/addgene:78897 ; RRID:Addgene_78897)

  • For your References section:

    In Vivo Transcriptional Activation Using CRISPR/Cas9 in Drosophila. Lin S, Ewen-Campen B, Ni X, Housden BE, Perrimon N. Genetics. 2015 Oct;201(2):433-42. doi: 10.1534/genetics.115.181065. Epub 2015 Aug 5. 10.1534/genetics.115.181065 PubMed 26245833
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