pET-TRSter
(Plasmid #78865)

• Purpose: Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element
• Depositing Lab: Elias Arnér
• Publication: Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.

Backbone

• Vector backbone: pET-24(s)+
• Backbone manufacturer: Novagen Inc.
• Total vector size (bp): 6796
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Expression host BL21(DE3)
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Thioredoxin Reductase
• Alt name: Txnrd1 (a.k.a. TR1 or TrxR1)
• Species: R. norvegicus (rat)
• Insert Size (bp): 3450
• GenBank ID: NM_031614.2
• Entrez Gene: Txnrd1 (a.k.a. Tr)
• Promoter: T7lac
• Tag / Fusion Protein:
  • Mutated E. coli-type SECIS element (3’ end of insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminal

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Additional article references: PubMed PMID: 15345395

A > G (nucleotide) at bp 4 compared to NM_031614.2, which results in N2D compared to NP_113802.2.