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Addgene

PA-RL-BAD
(Plasmid #78859)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78859 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone size w/o insert (bp) 5428
  • Modifications to backbone
    Protein A, Renilla luciferase, BAD inserted via LR reaction
  • Vector type
    Mammalian Expression ; Expression vector
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BCL2 associated agonist of cell death
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    530
  • GenBank ID
    NM_004322.3
  • Entrez Gene
    BAD (a.k.a. BBC2, BCL2L8)
  • Promoter CMV
  • Tag / Fusion Protein
    • Protein A, Renilla luciferase (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CMVF_pcDNA3, T7F
  • 3′ sequencing primer bGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PA-RL-BAD was a gift from Erich Wanker (Addgene plasmid # 78859 ; http://n2t.net/addgene:78859 ; RRID:Addgene_78859)

  • For your References section:

    DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for Interactome Mapping in Mammalian Cells. Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C, Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE. J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8. 10.1016/j.jmb.2015.08.003 PubMed 26264872
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