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pGEX-4T1-USP11
(Plasmid #78849)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78849 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    USP11
  • Species
    H. sapiens (human)
  • Entrez Gene
    USP11 (a.k.a. UHX1)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer pGEX 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T1-USP11 was a gift from Jaewhan Song (Addgene plasmid # 78849 ; http://n2t.net/addgene:78849 ; RRID:Addgene_78849)

  • For your References section:

    USP11-dependent selective cIAP2 deubiquitylation and stabilization determine sensitivity to Smac mimetics. Lee EW, Seong D, Seo J, Jeong M, Lee HK, Song J. Cell Death Differ. 2015 Sep;22(9):1463-76. doi: 10.1038/cdd.2014.234. Epub 2015 Jan 23. 10.1038/cdd.2014.234 PubMed 25613375
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