pET32a-His-FADD
(Plasmid
#78803)
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PurposeTo purify His-tagged recombinant FADD protein
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 78803 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-32a
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGIG3
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SpeciesH. sapiens (human)
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Entrez GeneFADD (a.k.a. GIG3, IMD90, MORT1)
- Promoter T7
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Tags
/ Fusion Proteins
- 6x His (N terminal on backbone)
- Thrombin cleavage site (N terminal on backbone)
- S-Tag (N terminal on backbone)
- TrxA (thioredoxin) (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET32a-His-FADD was a gift from Jaewhan Song (Addgene plasmid # 78803 ; http://n2t.net/addgene:78803 ; RRID:Addgene_78803)