pBABE-puro p21 K161Q, K163Q
(Plasmid
#78792)
-
PurposeRetroviral expression of p21 K161Q, K163Q
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 78792 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBABE-Puro
-
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameP21
-
SpeciesH. sapiens (human)
-
MutationK161Q, K163Q muation
-
Entrez GeneCDKN1A (a.k.a. CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1)
- Promoter 5'LTR
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE 3' (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBABE-puro p21 K161Q, K163Q was a gift from Jaewhan Song (Addgene plasmid # 78792 ; http://n2t.net/addgene:78792 ; RRID:Addgene_78792) -
For your References section:
Stabilization of p21 (Cip1/WAF1) following Tip60-dependent acetylation is required for p21-mediated DNA damage response. Lee MS, Seo J, Choi DY, Lee EW, Ko A, Ha NC, Yoon JB, Lee HW, Kim KP, Song J. Cell Death Differ. 2013 Apr;20(4):620-9. doi: 10.1038/cdd.2012.159. Epub 2012 Dec 14. 10.1038/cdd.2012.159 PubMed 23238566