pcDNA3-FLAG MKRN1 S109D
(Plasmid
#78775)
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PurposeTo overexpress MKRN1 S109D in Mammalian Cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 78775 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3-FLAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMKRN1
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SpeciesH. sapiens (human)
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MutationS109D_mutation
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Entrez GeneMKRN1 (a.k.a. RNF61)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-FLAG MKRN1 S109D was a gift from Jaewhan Song (Addgene plasmid # 78775 ; http://n2t.net/addgene:78775 ; RRID:Addgene_78775) -
For your References section:
PI3K/AKT activation induces PTEN ubiquitination and destabilization accelerating tumourigenesis. Lee MS, Jeong MH, Lee HW, Han HJ, Ko A, Hewitt SM, Kim JH, Chun KH, Chung JY, Lee C, Cho H, Song J. Nat Commun. 2015 Jul 17;6:7769. doi: 10.1038/ncomms8769. 10.1038/ncomms8769 PubMed 26183061