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pCS3-MT-BX/p14ARF
(Plasmid #78761)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78761 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS3-MT-BX-6xMyc
  • Backbone manufacturer
    derived from pCS2+
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ARF
  • Species
    H. sapiens (human)
  • Entrez Gene
    CDKN2A (a.k.a. ARF, CAI2, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16)
  • Promoter CMV IE94
  • Tag / Fusion Protein
    • 6xMYC (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS3-MT-BX/p14ARF was a gift from Jaewhan Song (Addgene plasmid # 78761 ; http://n2t.net/addgene:78761 ; RRID:Addgene_78761)

  • For your References section:

    Acceleration of gastric tumorigenesis through MKRN1-mediated posttranslational regulation of p14ARF. Ko A, Shin JY, Seo J, Lee KD, Lee EW, Lee MS, Lee HW, Choi IJ, Jeong JS, Chun KH, Song J. J Natl Cancer Inst. 2012 Nov 7;104(21):1660-72. doi: 10.1093/jnci/djs424. Epub 2012 Oct 25. 10.1093/jnci/djs424 PubMed 23104211
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