pcDNA3.nucGAP
(Plasmid
#78736)
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PurposeExpression of Ca2+ sensor GAP in the nucleus
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78736 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenucGAP
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SpeciesSynthetic
- Promoter CMV
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Tag
/ Fusion Protein
- nucleoplasmin (Xenopus) (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.nucGAP was a gift from Teresa Alonso (Addgene plasmid # 78736 ; http://n2t.net/addgene:78736 ; RRID:Addgene_78736) -
For your References section:
GAP, an aequorin-based fluorescent indicator for imaging Ca2+ in organelles. Rodriguez-Garcia A, Rojo-Ruiz J, Navas-Navarro P, Aulestia FJ, Gallego-Sandin S, Garcia-Sancho J, Alonso MT. Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2584-9. doi: 10.1073/pnas.1316539111. Epub 2014 Feb 5. 10.1073/pnas.1316539111 PubMed 24501126