pcDNA3.cytGAP
(Plasmid #78734)

• Purpose: Expression of Ca2+ sensor GAP in the cytosol
• Depositing Lab: Teresa Alonso
• Publication: Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2584-9. doi: 10.1073/pnas.1316539111. Epub 2014 Feb 5.

Backbone

• Vector backbone: pcDNA3
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cytGAP
• Species: Synthetic
• Promoter: CMV
• Tag / Fusion Protein:
  • luciferase (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GAP is a fusion of two Aequorea victoria proteins, GFP and apo-aequorin, with a linker in between. The excitation spectrum of GAP maintained the two peaks of wtGFP, at 403 and 470 nm, and it was sensitive to Ca2+.