pYN4+
(Plasmid #78715)

• Purpose: mammalian expression of NHE1
• Depositing Lab: Larry Fliegel
• Publication: Reddy et al J Biol Chem. 2008 Aug 8;283(32):22018-30. doi: 10.1074/jbc.M803447200. Epub 2008 May 28.

Backbone

• Vector backbone: pOPRSVICat
• Backbone manufacturer: Stratagene
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NHE1
• Species: H. sapiens (human)
• Insert Size (bp): 2500
• Mutation: D720G
• Entrez Gene: SLC9A1 (a.k.a. APNH, LIKNS, NHE-1, NHE1, PPP1R143)
• Promoter: RSV-LTR
• Tag / Fusion Protein:
  • HA3x (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (destroyed during cloning)
• 3′ cloning site: NotI (destroyed during cloning)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: TK-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the D720G mutation does not appear to affect protein function.