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p5E-acta2
(Plasmid #78686)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78686 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONRp4p1r
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2600
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    acta2 promoter
  • Alt name
    actin, alpha 2, smooth muscle, aorta
  • Alt name
    alpha smooth muscle actin
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    2165
  • GenBank ID
    NM_212620.1
  • Entrez Gene
    acta2 (a.k.a. wu:fb63d03)
  • Promoter acta2

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The entire intron 1 was inserted upstream of 300 bp of proximal acta2 promoter to make a compact promoter/enhancer construct that could easily be used for transgenesis.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5E-acta2 was a gift from Sarah Childs (Addgene plasmid # 78686 ; http://n2t.net/addgene:78686 ; RRID:Addgene_78686)

  • For your References section:

    An alpha-smooth muscle actin (acta2/alphasma) zebrafish transgenic line marking vascular mural cells and visceral smooth muscle cells. Whitesell TR, Kennedy RM, Carter AD, Rollins EL, Georgijevic S, Santoro MM, Childs SJ. PLoS One. 2014 Mar 3;9(3):e90590. doi: 10.1371/journal.pone.0090590. eCollection 2014. PONE-D-13-28889 [pii] PubMed 24594685
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