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pBS AluYa5rescueA70D
(Plasmid #78657)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78657 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene?
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 5374
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ya5rescue cassette
  • Alt name
    Alurescue
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2430

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Only when the Alu retrotransposes will the cells become neomycin resistant

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS AluYa5rescueA70D was a gift from Astrid Roy-Engel (Addgene plasmid # 78657 ; http://n2t.net/addgene:78657 ; RRID:Addgene_78657)

  • For your References section:

    Rescuing Alu: recovery of new inserts shows LINE-1 preserves Alu activity through A-tail expansion. Wagstaff BJ, Hedges DJ, Derbes RS, Campos Sanchez R, Chiaromonte F, Makova KD, Roy-Engel AM. PLoS Genet. 2012;8(8):e1002842. doi: 10.1371/journal.pgen.1002842. Epub 2012 Aug 9. 10.1371/journal.pgen.1002842 PubMed 22912586
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