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pCEPO2Ya5rescue
(Plasmid #78656)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78656 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCEP4.1
  • Backbone manufacturer
    Thermo Scientific
  • Backbone size w/o insert (bp) 9065
  • Total vector size (bp) 16348
  • Vector type
    Mammalian Expression ; Episomal
  • Selectable markers
    Neomycin (select with G418), Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Ya5rescue cassette
  • Alt name
    Alurescue
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2504

Gene/Insert 2

  • Gene/Insert name
    CMV-ORF2p-pA
  • Alt name
    L1 ORF2opt
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4737
  • Mutation
    L1 ORF2 codons have been "humanized"
  • Tag / Fusion Protein
    • myc-his (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Only when the Alu retrotransposes will the cells become neomycin resistant

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEPO2Ya5rescue was a gift from Astrid Roy-Engel (Addgene plasmid # 78656)

  • For your References section:

    SINE Retrotransposition: Evaluation of Alu Activity and Recovery of De Novo Inserts. Ade C, Roy-Engel AM. Methods Mol Biol. 2016;1400:183-201. doi: 10.1007/978-1-4939-3372-3_13. 10.1007/978-1-4939-3372-3_13 PubMed 26895055
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