pBW204J115-gfp
(Plasmid
#78643)
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PurposepSB3K3 carrying J115-30gfp-t
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 78643 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSB3K3
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Backbone manufacturerBioBrick vector, IGEM registry
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameJ115-30gfp-t
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SpeciesSynthetic
- Promoter see insert
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBRforEco
- 3′ sequencing primer RNAde-BB-2R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBW204J115-gfp was a gift from Baojun Wang (Addgene plasmid # 78643 ; http://n2t.net/addgene:78643 ; RRID:Addgene_78643) -
For your References section:
Engineering modular and tunable genetic amplifiers for scaling transcriptional signals in cascaded gene networks. Wang B, Barahona M, Buck M. Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593. Epub 2014 Jul 16. 10.1093/nar/gku593 PubMed 25030903