pBW102ParsR-Amp32C
(Plasmid
#78637)
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PurposepBW100ParsR carrying 30hrpR-32hrpS-t-hrpL-30gfp-t
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 78637 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBW100ParsR
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Backbone manufacturerWang Lab
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert name30hrpR-32hrpS-t-hrpL-30gfp-t
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SpeciesSynthetic
- Promoter see insert
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBRforEco
- 3′ sequencing primer RNAde-BB-2R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBW102ParsR-Amp32C was a gift from Baojun Wang (Addgene plasmid # 78637 ; http://n2t.net/addgene:78637 ; RRID:Addgene_78637) -
For your References section:
Engineering modular and tunable genetic amplifiers for scaling transcriptional signals in cascaded gene networks. Wang B, Barahona M, Buck M. Nucleic Acids Res. 2014 Aug;42(14):9484-92. doi: 10.1093/nar/gku593. Epub 2014 Jul 16. 10.1093/nar/gku593 PubMed 25030903