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pTol2-DRv7
(Plasmid #78627)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78627 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Tol2
  • Backbone size w/o insert (bp) 12262
  • Total vector size (bp) 12586

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GESTALT lineage tracing target V7
  • Alt name
    V7
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    324

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GAATTCTCGACCTCGAGACAAATGGCAG
  • 3′ sequencing primer AAGCTTGAGCTCGAGATCTGAGTCCGGA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GESTALT V7 barcode for integration into zebrafish using the Tol2 system

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTol2-DRv7 was a gift from Jay Shendure (Addgene plasmid # 78627 ; http://n2t.net/addgene:78627 ; RRID:Addgene_78627)

  • For your References section:

    Whole organism lineage tracing by combinatorial and cumulative genome editing. McKenna A, Findlay GM, Gagnon JA, Horwitz MS, Schier AF, Shendure J. Science. 2016 May 26. pii: aaf7907. 10.1126/science.aaf7907 PubMed 27229144
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