pcDNA 3.1(+) eGFP p6 K27R
(Plasmid
#78583)
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PurposeExpresses GFP p6 with K27R mutation in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 78583 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA 3.1(+)
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 6278
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeGFP p6
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SpeciesH. sapiens (human)
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Insert Size (bp)850
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MutationHIV-1 HXB2 p6 with conservative mutations (G78A, G81A, G84A, G87A); K27R
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA 3.1(+) eGFP p6 K27R was a gift from Jeremy Luban (Addgene plasmid # 78583 ; http://n2t.net/addgene:78583 ; RRID:Addgene_78583) -
For your References section:
Covalent modification of human immunodeficiency virus type 1 p6 by SUMO-1. Gurer C, Berthoux L, Luban J. J Virol. 2005 Jan;79(2):910-7. 10.1128/JVI.79.2.910-917.2005 PubMed 15613319