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PurposeExpresses untagged human rhinovirus 3C protease in E. coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78571 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET42
- Total vector size (bp) 6358
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructionsExpress in BL21(DE3) cells
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namehuman rhinovirus 3C protease
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SpeciesH. sapiens (human)
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Entrez GeneHRVBgp1 (a.k.a. HRVBgp1)
- Promoter T7
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSRK2706 was a gift from David Waugh (Addgene plasmid # 78571 ; http://n2t.net/addgene:78571 ; RRID:Addgene_78571) -
For your References section:
A dual protease approach for expression and affinity purification of recombinant proteins. Raran-Kurussi S, Waugh DS. Anal Biochem. 2016 Apr 19;504:30-37. doi: 10.1016/j.ab.2016.04.006. 10.1016/j.ab.2016.04.006 PubMed 27105777
Map uploaded by the depositor.
