pCVD502
(Plasmid
#78570)
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PurposeA 5.6 kb Sa1I fragment carryig the 1st tdh gene copy was cloned from pCVD501 into the Sa1I site of pBR322.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 78570 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
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Vector typeCloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametdh1
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SpeciesVibrio parahaemolyticus
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pBRforBam
- 3′ sequencing primer Tet-internal-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCVD502 was a gift from James Kaper (Addgene plasmid # 78570 ; http://n2t.net/addgene:78570 ; RRID:Addgene_78570) -
For your References section:
Nucleotide sequence of the thermostable direct hemolysin gene of Vibrio parahaemolyticus. Nishibuchi M, Kaper JB. J Bacteriol. 1985 May;162(2):558-64. PubMed 3988703