pCAM9
(Plasmid
#78560)
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PurposepBAD30::ompU V. cholerae 395 Induce with 0.2% arabinose. Expression system: V. cholerae 395 outer membrane protein OmpU.
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
Availability may be limited outside the U.S. Please log in for more information.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78560 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAD30
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameompU
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SpeciespCAM9
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Insert Size (bp)3159
- Promoter araBAD promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBAD-F
- 3′ sequencing primer pBAD-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAM9 was a gift from James Kaper (Addgene plasmid # 78560 ; http://n2t.net/addgene:78560 ; RRID:Addgene_78560) -
For your References section:
The ToxR-mediated organic acid tolerance response of Vibrio cholerae requires OmpU. Merrell DS, Bailey C, Kaper JB, Camilli A. J Bacteriol. 2001 May;183(9):2746-54. 10.1128/JB.183.9.2746-2754.2001 PubMed 11292792