LC-E03
(Bacterial strain #78552)

• Purpose: E. coli strain K-12 MG1655, pTet--Cas9 cassette integrated at 186 primary attB site.
• Depositing Lab: David Bikard
• Publication: Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw223.

Backbone

• Vector backbone: na

Growth in Bacteria

• Bacterial Resistance(s): None
• Growth Temperature: 37°C
• Growth Strain(s): LC-E03
• Growth instructions: 37°C, aerobic growth, shaking (optional), no illumination required. The viability can be checked by making a liquid culture (or grow in solid media) in the culturing condition described above.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pTet--Cas9 cassette integrated at 186 primary attB site
• Mutation: This is a bacterial strain

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: na

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pOSIP (St-Pierre, 2013, ACS Synth. Bio) and pwtCas9-bacteria (Qi, 2013, Cell)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry