pJR255
(Plasmid #78549)

• Purpose: (Empty Backbone) For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. CMV driven mCherry visible marker.
• Depositing Lab: John Rossi
• Publication: John Rossi lab small RNA expression plasmids (unpublished)

Backbone

• Vector backbone: peGFP-N3
• Backbone manufacturer: Clontech
• Backbone size (bp): 5025
• Modifications to backbone: Replace eGFP by mCherry . Silent mutation at K143 of mCherry to remove Bbs-1 site. Added U6 promoter with two Bbs-1 sites.
• Vector type: Mammalian Expression, RNAi
• Promoter: U6, CMV
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry