pLentiCas9-T2A-BFP Notes
Addgene Diagnostic Digest
This gel depicts the NEB 1kb extended ladder and a digestion of the plasmid pLentiCas9-T2A-BFP with the following enzymes: NheI-HF, NdeI, and EcoRV-HF.
Expected Bands
NheI-HF: 13.6 kb
NheI-HF/ NdeI: 11.5 kb & 2.1 kb
EcoRV-HF: 9.1 kb, 3.6 kb, 0.8 kb, & 0.08 kb (off gel)
We observe the expected bands for this digest.
