pLentiCas9-T2A-BFP
(Plasmid #78547)

• Purpose: Cas9 protein with BFP
• Depositing Labs: Roderic Guigo, Rory Johnson
• Publication: Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005341. doi: 10.1371/journal.pcbi.1005341. eCollection 2017 Mar.

Backbone

• Vector backbone: Lenti-Cas9-blast
• Backbone manufacturer: Addgene 52962
• Backbone size w/o insert (bp): 12859
• Total vector size (bp): 13633
• Modifications to backbone: Added T2A-BFP
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: T2A-BFP
• Insert Size (bp): 753
• Promoter: In frame with Cas9

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CTGGACGCCACCCTGATCC
• 3′ sequencing primer: Gacgctgtagtcttcagagatg

Resource Information

• Supplemental Documents:
  • pLenti-Cas9-T2A-GFP
• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry